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Analyze: Hybridization Time & Concentrations
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Analyze: Hybridization Time & Concentrations
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This window displays hybridization times for oligos of various lengths and in various concentrations.
Half Hybridization Time is calculated according to Keller, G.H. (1993) DNA Probes: Molecular Hybridization Technology, pp. 1-25; Stockton Press. T1/2 = sqrt N * ln 2 / (350000 * C) where N is the oligo length [nt] and C, its concentration [mol/L]. This formula is good for standard hybridization conditions with salt concentration range from 0.4 to 1 M NaCl. |
The Concentrations window is a nucleic acid calculator. It provides instant concentration, volume, absorption and molecular weight conversions for your primers, PCR products and DNA templates with a click of the mouse.
