This window displays hybridization times for oligos of various lengths and in various concentrations.
Half Hybridization Time is calculated according to Keller, G.H. (1993) DNA Probes: Molecular Hybridization Technology, p. 1-25; Stockton Press.
T1/2 = sqrt N * ln 2 / 350000 * C
where N is the oligo length [nt] and C, its concentration [mol/L]. Formula good for standard hybr. conditions at 0.4-1 M NaCl.
