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Summary
These were the main features of OLIGO. The software has been widely recognized by a scientific community, and became a standard in its class. Since 1989 about five thousand of copies have been sold.
It has all the features required for optimal design of multiplex and consensus PCR primers, TaqMan and in situ hybridization probes, guess-mer probes and the least degenerate primers & probes. Its reverse-translation, hypothetical restriction sites search in protein and the secondary structure search features make it the ideal tool for designing genes and site-directed mutagenesis experiments. It gives hybridization times, re-calculates nucleic acids concentrations and absorptions making design of your experiment an easy and even sometimes enjoyable task.
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