Primer Pairs & Selected Oligos
OLIGO's search for sequencing primers accounts for the following parameters: 3' dimers and hairpins (measured by both structure and stability), length, GC clamps, false priming (in the template and in other sequence files), homooligomers/sequence repeats and melting temperatures. More sub-search options are engaged when searching for PCR primer pairs. The lists of search results are shown below.