Oligonucleotide Database
Any number of oligonucleotide databases may be created in OLIGO, each with a 25,000+ storage capacity. Determine priming efficiency, dimers and multiple exon (gene) multiplexing by using one of OLIGO's database files. Data from an oligonucleotide database may also be transferred directly to an order form to be e-mailed or faxed to your synthesis facility.
The ID Number is generated automatically. It consists of the accession number, position of the first (the lowest position number) nucleotide in the primer on the sequence, letter "L" for a negative strand or "U" for a positive strand primer, and the primer length. In the "3' dim. DeltaG" field you can read two types of data: 1) free energy of the 3'-end dimer (if you multiplex using this window, this value refers to the strongest dimer formed with any of the multiplexed primers; 2) a letter code for compatibility & multiplexing: M- a multiplexed primer (compatible with all M and C primers), C- compatible primer with any multiplexed oligo, D- primer forming a self-dimer (the stringency definition can be set by changing the search parameters), NC- primer that is compatible with itself but not compatible with at least one multiplexed primers. P.E.- priming efficiency numbers: first the actual number within a user-selected search range, second the maximum value (with no mismatches).